Pep Guardiola‘s men travel to the Spanish capital for the first leg, which gets under way at 20:00 (UK) on Tuesday 9 April.
The two sides locked horns in the last four of the competition in 2022/23, with City running out 5-1 winners on aggregate thanks to an incredible second leg display at the Etihad Stadium.
At the Santiago Bernabeu, Kevin De Bruyne‘s second half strike had been enough to cancel out Vinicius Junior’s solo effort in a thrilling 1-1 draw.
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But two of Europe’s heavyweights will now do battle once again, with a spot in the semis up for grabs.
City come into the tie on the back of a 4-1 Premier League win over Crystal Palace but Madrid, unbeaten since January in all competitions, are in equally good form ahead of our latest showdown.
In preparations for that clash, Guardiola‘s men were put through our paces before making the journey to Spain.
Watch a section of the session via the video above...