The centre back picked up an ankle injury during City’s pre-season match against Sunderland in Hong Kong at the end of last month.
With Vincent Kompany also injured, the manager called on Javi Garcia to fill the void in the heart of defence for the opening two fixtures.
“Matija is available to play tomorrow,” the Chilean revealed.
“I think Javi Garcia didn’t do a bad job as he normally plays in midfield. But it is Nastasic’s natural position, so of course it is better we have him there.”
The Blues boss also confirmed injuries will keep Kompany and Micah Richards out of action for the second home game of the season.
“Nothing is new with Vincent, “he said.
“[At the time] the doctor said it will be four to six weeks. Maybe next week we will see how he improves, but less than one month is impossible.
“Micah is not available for this game, but I hope that next Monday we will start working normally with him.”
The morning after City learnt their Champions’ League group stage fate, Pellegrini declared group D would provide a tough test, but is confident of making it to the next stage of the competition.
“I think all are difficult groups, maybe some have more history in the Champions League than others, but all are difficult,” he stated.
We have a tough group, but I think we have a chance to go through to the round of 16 if we play well.
...Manuel Pellegrini...
“It’s one of our targets to qualify for the next round and I hope we can go further in the tournament this year.”
Following City’s disappointing defeat at the hands of Cardiff City last week, the manager stated he is keen to get back to winning ways against Hull City.
The Blues conceded two goals from set pieces in south Wales and he admitted the team has been working hard to strengthen this area.
“Set pieces are very important and we must learn from last week,” he said.
“It was a bad game for us in that way, but I we must improve for our next game.
“I think Hull have started well, they played very well against Chelsea. We will have to mark a very difficult team here also, so we will have to play very well.”