Vincent Kompany remains out, as does long-term absentee Claudio Bravo, but the rest of the first-team squad are available for selection.
And after playing five academy players in the 1-0 win over Burton Albion in the Carabao Cup semi-final on Wednesday, Guardiola says they will not be involved when Sean Dyche’s side visit the Etihad as he looks to play his strongest squad in a bid to remain in all four competitions.
“They [academy players] have to be prepared, just in case,” he said.
“Now, except Vinny, the other ones are fit and everybody can play.
“Tomorrow there will be no players from the academy.
“I always try to put out a strong team. It’s FA Cup against Premier League side like Burnley so we will play with the best team we believe to win the game.
“They players are quite well. We will see today [at training].
“We will make regeneration. Some players have a few problems but I think they will be okay.”
WATCH: Burton 0-1 City | Extended Highlights
READ: Touaizi | Garcia’s form a boost for academy lads
Benjamin Mendy returned to action in the win at the Pirelli Stadium, playing 30 minutes after returning from a knee injury that saw him sidelined for 10 weeks.
He came through the game unscathed and is available for Saturday’s game - but Guardiola refused to say whether the Frenchman would be ready to play from the start.
“it was the first 25minutes after a long time, so we will see,” he said.