Interim head coach Lee Carsley has included John Stones, Rico Lewis, Phil Foden and Jack Grealish in his maiden roster for matches with the Republic of Ireland and Finland.
The Three Lions will be aiming to gain promotion back into League A from Group B2, which also contains Greece, across the latest tournament.
This starts with a trip to the Aviva Stadium on Saturday 7 September, with kick-off against Heimir Hallgrimsson’s side set for 17:00 (UK).
England then face Finland at Wembley at 19:45 (UK) on Tuesday 10 September.
The first round of Nations League fixtures represents the Three Lions’ first matches since reaching the final of Euro 2024 where they narrowly lost 2-1 to Spain.
Since then, Gareth Southgate had departed as head coach and Carsley was promoted from the Under-21s to temporary charge of the senior team.
The latter’s squad is as follows...
ENGLAND SQUAD
Goalkeepers: Dean Henderson (Crystal Palace), Jordan Pickford (Everton), Nick Pope (Newcastle).
Defenders: Trent Alexander-Arnold (Liverpool), Levi Colwill (Chelsea), Marc Guehi (Crystal Palace), Ezri Konsa (Aston Villa), Tino Livramento (Newcastle), Rico Lewis (Manchester City), Harry Maguire (Manchester United), John Stones (Manchester City).
Midfielders: Phil Foden (Manchester City), Conor Gallagher (Atletico Madrid), Angel Gomes (Lille), Kobbie Mainoo (Manchester United), Cole Palmer (Chelsea), Declan Rice (Arsenal), Morgan Gibbs-White (Nottingham Forest).
Forwards: Jarrod Bowen (West Ham), Eberechi Eze (Crystal Palace), Anthony Gordon (Newcastle), Jack Grealish (Manchester City), Harry Kane (Bayern Munich), Noni Madueke (Chelsea), Bukayo Saka (Arsenal), Ollie Watkins (Aston Villa).
