The Three Lions take on Wales in a friendly on 9 October (19:45 UK) before facing Latvia in a World Cup qualification clash on Tuesday 14 October, also at 19:45 (UK).
Both City stars were part of Tuchel’s last England squad, but Stones, who has so far won 83 caps for his country, was forced to withdraw with a minor muscle issue.
Trafford is yet to win a full senior cap for England.
Full squad
Goalkeepers: Dean Henderson (Crystal Palace), Jordan Pickford (Everton), James Trafford (Manchester City)
Defenders: Dan Burn (Newcastle United), Marc Guéhi (Crystal Palace), Reece James (Chelsea), Ezri Konsa (Aston Villa), Myles Lewis-Skelly (Arsenal), Jarell Quansah (Bayer Leverkusen), Djed Spence (Tottenham Hotspur), John Stones (Manchester City)
Midfielders: Elliot Anderson (Nottingham Forest), Morgan Gibbs-White (Nottingham Forest), Jordan Henderson (Brentford), Ruben Loftus-Cheek (AC Milan), Declan Rice (Arsenal), Morgan Rogers (Aston Villa)
Forwards: Jarrod Bowen (West Ham United), Eberechi Eze (Arsenal), Anthony Gordon (Newcastle United), Harry Kane (Bayern Munich), Marcus Rashford (Barcelona, loan from Manchester United), Bukayo Saka (Arsenal), Ollie Watkins (Aston Villa)
