Kyle Walker and John Stones have both been ruled out of the trip to Elland Road, though Joao Cancelo is available again after serving a one-match Champions League ban.
Asked if this game had come too soon for Walker, the City boss said: “Yes.”
He was then asked if he might be back before the end of the season or miss the remaining games.
Pep replied: “I don’t know right now. Right now it’s no worry at all - I count on the guys who are fit.
“He has to work to come back as soon as possible. John (Stones) is out tomorrow - we will see (how he is) for Wednesday.”
With Liverpool kicking off at lunchtime on Saturday, City could start the game against Leeds in second position.
Though the Blues thrashed Leeds 7-0 earlier in the season, Guardiola says he expects a difficult 90 minutes against a side that are unbeaten in their last five and fighting relegation.
“Always when I play against Leeds it is difficult,” said Pep.
“Last season we dropped five points against them. Jesse Marsch made a great job in Salzburg, the US, Leipzig - it’s perfect for him.
“Many teams in England are playing this way. I have a lot of respect. It’s between the Champions League semi-finals, so maybe it’s one of the toughest.
“It’s tougher because Leeds is tough. I know how the crowd is, they are fighting because they need points. We need points too. I saw the team today and they are incredibly focused.
“Everyone in the club knows what we are playing for. We know what we have to do.
“We make an incredible last three games, scored a lot of goals and created chances - this is what we have to continue to do.
“Sometimes in football you have rewards, other times defeats.
“This is the most important game we have. After Madrid, if we are in the final or not, we have four games and we can focus on the Premier League.
“We have spoken about that. We spoke about what a special team they are and we accept the challenge, we know what we have to do and we will try to do it.”
