City duo Ruby Mace and Laura Blindkilde Brown both featured for England under-23s in a 3-1 defeat against Spain.
Mace started the game in Marbella as the Young Lionesses made a strong start with Aston Villa’s Freya Gregory and Chelse striker Aggie Beever-Jones both hitting the crossbar.
England took the lead in the 42nd minute when Tottenham’s Jessica Naz pounced on a loose pass to tee up Beever-Jones for a simple finish.
But Spain went in level at half-time following a quick equaliser from Olhane Valdezate and took control after the break.
Inmaculada Gabarro missed a penalty but Bruna Vilamala struck twice to seal the victory.
City’s January signing Blindkilde Brown came off the bench in the 73rd minute as England tried to find a way back into the game.
England will next play Netherlands on Monday 26 February at 12:00 (UK).
