Ellie Roebuck, Alex Greenwood, Esme Morgan, Laura Coombs, Lauren Hemp, Chloe Kelly and Jess Park were all called up on Wednesday by England boss Sarina Wiegman.
The Lionesses will be in action for the first time since reaching the World Cup final in Australia last month where they were narrowly beaten by Spain.
Drawn in Group A1 along with the Netherlands, Belgium and Scotland, England begin their Nations League campaign against the latter on Friday 22 September at the Stadium of Light in Sunderland at 19:45 (UK).
They then travel to Utrecht to face Netherlands on Tuesday 26 September at 19:00 (UK) with City duo Kerstin Casparij and Jill Roord in the Dutch squad.
We will have a City-focused report available to view on both matches on mancity.com and the official Man City app soon after the final whistle.
England squad
Goalkeepers
Mary Earps
Hannah Hampton
Defenders
Millie Bright
Lucy Bronze
Jess Carter
Niamh Charles
Maya Le Tissier
Lotte Wubben-Moy
Midfielders
Jordan Nobbs
Lucy Staniforth
Georgia Stanway
Ella Toone
Katie Zelem
Forwards
Rachel Daly
Lauren James
Alessia Russo
Katie Robinson
