Ben Wilkinson’s side welcome the Royals to the City Football Academy on Wednesday 14 February, with kick-off scheduled for 19:00 (UK).
Our youngsters reached the fifth round of the historic competition with a strong 2-0 victory away at holders West Ham United in January following extra-time.
CITY V READING FA YOUTH CUP | WATCH LIVE ON CITY+
Reading, meanwhile, secured passage into the last-16 thanks to an emphatic 4-0 success on the road against Sheffield Wednesday.
Last term, City suffered semi-final heartbreak in a 2-1 defeat to Arsenal at Emirates Stadium when the Gunners took the lead deep into extra-time.
But how will our latest assignment in the FA Youth Cup play out as we aim to reach the quarter-finals?
CITY+ subscribers can watch the action unfold on mancity.com and our official app with the broadcast starting shortly before the 19:00 (UK) start.
CITY+
CITY+ is available via an annual subscription of £34.99 a year, saving you £24 on our monthly offering.
Choose monthly and pay £4.99 a month with the option to cancel anytime.
Official Cityzens and Season Ticket Members can subscribe annually for £30 a year, a saving of £29.