The Argentine midfielder has agreed to join the La Liga side until June 2015.
Zuculini, 21, signed for City earlier this summer from Racing Club in his homeland, and caught the eye on pre-season tour, featuring six times for scoring once against Kansas City.
He was rewarded for his impressive showings in Scotland and the USA with a substitute appearance in the Community Shield against Arsenal at Wembley.
The highly-rated Argentine youth international scored 10 goals in 92 appearances for Racing after making his senior debut at the tender age of 17, and has eight caps for Argentina at u20 level.
Valencia kick off their La Liga campaign against Sevilla on Saturday.
We’ll keep you updated with how Bruno gets on in Spain in our weekly loan watch feature here on mcfc.co.uk.
