Goalkeeper Ellie Roebuck, skipper Steph Houghton, Demi Stokes, Georgia Stanway and Keira Walsh, who were all part of the squad which reached the World Cup semi-finals in the summer, have been included.
And there is a first senior call-up for defender Aoife Mannion, who joined City this summer.
Jill Scott has been rested after her outstanding World Cup campaign in order to manage the City midfielder’s load ahead of the start of the new FA Women’s Super League season. Team-mates Ellen White and Karen Bardsley miss out due to injury.
There are also first senior calls for goalkeeper Sandy MacIver, defender Anna Patten and striker Beth England.
The 25-strong Lionesses face Belgium at the Den Dreef Stadium in Leuven on Thursday, 29 August (kick off 6.30pm BST)
The squad then travel to Bergen to take on their World Cup quarter-final opponents Norway at Brann Stadium on Tuesday 3 September (kick off 5pm BST).
England manager Phil Neville said: “After a strong performance at the World Cup, but one which ultimately didn’t quite bring the success we had hoped for, it’s important that we now regroup and refocus on our next targets.
“They are to join forces with the home nations as part of Team GB’s 2020 Olympics campaign and, ultimately, to be ready to win Euro 2021 on home soil in two years’ time.
“I’m delighted to be able to give Beth, Aoife, Anna and Sandy their first senior call-ups.”
Lionesses squad
Goalkeepers: Mary Earps (Manchester United), Sandy MacIver (Clemson University), Ellie Roebuck (Manchester City), Carly Telford (Chelsea)
Defenders: Millie Bright (Chelsea), Rachel Daly (Houston Dash), Alex Greenwood (Lyon), Steph Houghton (Manchester City), Aoife Mannion (Manchester City), Abbie McManus (Manchester United), Anna Patten (University of South Carolina), Demi Stokes (Manchester City), Leah Williamson (Arsenal)
Midfielders: Lucy Bronze (Lyon), Jade Moore (Reading), Lucy Staniforth (Birmingham City), Georgia Stanway (Manchester City), Keira Walsh (Manchester City)
Forwards: Toni Duggan (Atletico Madrid), Bethany England (Chelsea), Fran Kirby (Chelsea), Beth Mead (Arsenal), Nikita Parris (Lyon), Jodie Taylor (Seattle Reign).
