City are currently a point behind leaders Arsenal with a game in hand ahead of the match at the Etihad Stadium at 17:30 (UK).
We won all our five Premier League matches in April to keep the pressure on the Gunners, including a 2-0 victory at Nottingham Forest last Sunday.
Ruben Dias and Phil Foden were both missing through illness while goalkeeper Ederson was replaced at half-time following a clash with Forest defender Willy Boly.
Guardiola confirmed that the three will be available for this week’s game.
“[Foden and Dias are] okay,” he said in his pre-match news conference.
On Ederson, he added: “It looked worse than it is. He trained yesterday and feels good.”
Erling Haaland was a second half substitute in the victory over Forest, coming off the bench to score the crucial second goal.
The Norwegian striker had missed the FA Cup semi-final victory over Chelsea and the 4-0 win away to Brighton with a muscle injury.
And Guardiola confirmed he is ready to start the game against Wolves on Saturday.
“When everyone is fit they have to be ready,” he added.
“I don’t exactly know what they will do tomorrow. We need to see the last games from Wolves.”
City had a rare midweek free of games following our victory over Forest on Sunday.
Guardiola believes it will help with the mental and physical freshness of his players for the final games of the season, with four Premier League matches remaining and an FA Cup final against Manchester United to come.
“[We had] days off and three really good training sessions, as always,” he said.
“Fresh the mind and tactics with little details. But at this stage there is not much to talk about tactics.
“Fresh minds and get legs ready for the game tomorrow.”